Biomimbone

RESULTS

- Studies and analysis regarding bone addition materials used in reconstructive surgery, natural used biomaterials and their processing technologies for customized biomimetic implants obtaining.
- Experimental development of innovative technologies, the obtaining of new solutions for synthesis methods of materials for biomimetic implants. Complex characterization and laboratory testing.
- Production of three-dimensional biomimetic test samples from pastes and / or powders and processing of massive components. In vitro biocompatibility study by standardized methods.

ACTIVITIES

- Identification of the main requirements and limits regarding customized biomimetic implants design for major bone defects reconstruction and their testing and sterilization methods; the existing domain norms analysis.
- The performances analysis of commercially available bone addition products.
- The analysis and classification of bone reconstruction rectified defects by dimensional, topological and morphological limits.
- Obtaining of primary apatite materials by thermal methods deproteinization.
- The obtaining of apatite biomaterials through thermal processing with different parameters (temperatures 700-1300oC and maintaining for structural transformation) for the obtaining of optimum characteristics and properties (the conversion of programmed HA in and -TCP).
- Characterization and complex testing, [composition by EDS+XRF, structure by XRD and FTIR, morphology by SEM and AFM, and mechanical properties] selection and classification of biomimetic apatite biomaterials obtained in different compositional versions for major bone deffects reconstruction. The obtaining of a diagram of biomaterials characteristics according to the synthesis route.
- Natural origin biomaterials processing and selection for the obtaining of apatite powders with predetermined characteristics (HA/TCP ratio, granulometry sorting) processing for synthesis of monodisperse powders and/or pastes.
- Samples analysis and further selection of the optimum for in vitro testing.
- Qualitative and quantitative in vitro quantitative and qualitative assessment of cell viability and selection of apatite powders with optimal properties for obtaining three-dimensional shapes.
- Establishing the sterilization and packing procedure for biomimetic apatite products.
- Computer design of three-dimensional shape and model in spatial coordinates.
- Obtaining three-dimensional test samples from monodispersion and / or paste with variable HA / TCP ratio and predetermined particle size; parameterization of practical experiments.
- Complex analysis on test samples of composition, morphology, structure and microdurity; demonstration of reproducibility (composition by EDS + XRF, structure by XRD and FT-IR, morphology by SEM and determination of microdurity).
- Reconsidering the steps of obtaining materials for biomimetic products for the reconstruction of major flaws and obtaining a characterization chart.
- Assessing the compliance of three-dimensional models obtained with computer-aided models.
- Experimenting the obtainedproducts and integrating them into the research laboratory.
- Linking to research structures the results of obtaining apatite materials obtained by presenting the results at conferences.
- Elaboration of the technological scheme and large-scale dissemination by publishing articles in ISI journals.
- Dissemination by communication and publication of the results.

Articles

^{Journal research article - ISI Indexed - IF 5,95}
ACS Sustainable Chemistry and Engineering, 2017
Progress in Hydroxyapatite - Starch Based Sustainable Biomaterials for Biomedical Bone Substitution Applications.

^Authors
F. Miculescu, A. Maidaniuc, SI Voicu, Vijay Kumar Thakur, G. E. Stan, L. T. Ciocan

^DOI http://dx.doi.org/10.1021/acssuschemeng.7b02314

^{Journal research article - ISI Indexed - IF 3,387}
Applied Surface Science, 2017
Induced wettability and surface-volume correlation of composition for bovine bone derived hydroxyapatite particles.

^Authors
A. Maidaniuc, F. Miculescu, S.I. Voicu, C. Andronescu, M. Miculescu, E. Matei, A.C. Mocanu, I. Pencea, I. Csaki, T. Machedon-Pisu, L.T. Ciocan

^DOI http://dx.doi.org/10.1016/j.apsusc.2017.07.074

^{Journal research article - ISI Indexed - IF 1,53}
Vacuum, 2017
Facile synthesis and characterization of hydroxyapatite particles for high value nanocomposites and biomaterials.

^Authors
F. Miculescu, A.C. Mocanu, C.A. Dascalu , A. Maidaniuc, D. Batalu, A. Berbecaru, S.I. Voicu, M. Miculescu, V. Kumar Thakur, L.T. Ciocan

^DOI http://dx.doi.org/10.1016/j.vacuum.2017.06.008

^{Journal research article - ISI Indexed}
UPB Scientific Bulletin, Series B: Chemistry and Materials Science, Volume 79, Issue 1, 2017, Pages 145-154, WOS:000405772200015
Sinterability study of bovine-derived hydroxyapatite and silver microcomposites.

^Authors
1. A Maidaniuc, F. Miculescu, A.C. Mocanu, S.I. Voicu, M. Miculescu, A. Purcaru, A. Muhule , C. Pop, M.E. Rada

^{Journal research article - ISI Indexed}
Journal Of Adhesion Science And Technology Vol.30, Iss.17, 2016
Effect of micron sized silver particles concentration on the adhesion induced by sintering and antibacterial properties of hydroxyapatite microcomposites.

^Authors
A. Maidaniuc, M. Miculescu, S. I. Voicu, L. T. Ciocan, M. Niculescu, M. C. Corobea, M. E. Rada & F. Miculescu

^DOI http://dx.doi.org/10.1080/01694243.2016.1163808

^{Conference article - BDI Indexed (Scopus)}
Advanced Materials - TechConnect Briefs 2016 Vol.3, pages 31-34, ISBN: 978-099751172-7
Strategies for production of naturally-derived calcium phosphates particles.

^Authors
Miculescu, F. , Maidaniuc, A. , Voicu, S.I. , Miculescu, M. , Batalu, D.

Volume

Book chapters

Chapter 7 Tuning Hydroxyapatite Particles Characteristics for Solid Freeform Fabrication of Bone Scaffolds (pp. 321-398) Authors F. Miculescu, A. Maidaniuc, G.E. Stan, M. Miculescu, S.I. Voicu, A. Cîmpean, V. Mitran and D. Batalu CNCS - UEFISCDI, project number PN-II-RU-TE-2014-4-0590 financial support is acknowleged Product page (external link to Wiley)

Invited paper presentation 1

^Title
Thermal processing routes for tunable calcium phosphates derived from bovine bone

^{Presented at the}
7^th International Conference “Biomaterials, Tissue Engineering & Medical Devices” BIOMMEDD 2016 in Constanta (Romania)

^Date
September 15^th to 18^th, 2016

^Summary
The presentation summarized the author's research regarding the preparation of tunable calcium phosphates through thermal processing of different types of bovine bone. Bovine bone-derived hydroxyapatite is considered and ecological and economical alternative to synthetic hydroxyapatite, with favourable sintering outcomes. The material is initially obtained after thermal treatments for removal of the bone's organic component, while preserving the mineral component's porous morphology. The information is centred on the variation of heat treatments parameters (temperature, environment and cooling conditions) and sintering strategies, and their effects upon the elemental and phase composition, morphology, structure, mechanical properties and thermal stability of the produced ceramics. Starting with precursor's selection, after material characterization and implantation studies, and up to powder processing for solid freeform fabrication, the presentation will outline the major achievements and challenges in thermal processing of bovine bone for its use in bone replacement products.

CNCS - UEFISCDI, project number PN-II-RU-TE-2014-4-0590 financial support is acknowleged

Invited paper presentation 2

^Title
Sintering strategies for bovine bone-derived hydroxyapatite and its microcomposites

^{Presented at the}
7^th International Conference on Materials Science and Technologies – RoMat 2016, Bucharest (Romania)

^Date
November 9^th to 11^th, 2016

^Summary
The presentation offers an evaluation of sintering strategies and additives used for improving the characteristics of bovine bone derived-hydroxyapatite and its microcomposites. Some materials adjustments are performed from the initial processing stages, through a careful management of heat treatments parameters. However, after the processing, further treatments (such as sintering or surface treatments) may be employed for enhancing material properties. In this case, the type and quality of the additives used for the treatments are crucial parameters, especially since these ceramics are designed for medical applications. This evaluation uses results from complementary material characterization techniques, which aim to study material thermal degradation (TGA-DSC), elemental chemical composition (EDS, XRF), morphology (SEM), structure and phase composition (XRD, FT-IR), and mechanical properties (hardness and compressive testing). These results and their correlation with the requirements of additive manufacturing techniques may be exploited for adapting the classic ceramic processing for preparing innovative products for bone replacement.

CNCS - UEFISCDI, project number PN-II-RU-TE-2014-4-0590 financial support is acknowleged

Conferences poster presentations

^{Presented at the}
7^th International Conference “Biomaterials, Tissue Engineering & Medical Devices” BIOMMEDD 2016 in Constanta (Romania)



^{Presented at the}
6^th International Conference on Materials Science and Technologies – RoMat 2016, Bucharest (Romania)



^{Presented at the}
18^th annual Nanotech 2016 Conference & Expo, co-located with the TechConnect World Innovation Conference, National Innovation Summit and National SBIR/STTR Conference, Washington DC, USA